Effect of phytoestrogen-rich fraction of Millettia aboensis on lipid profile, oxidative stress, and platelet count in ovariectomized rat model of menopause

Milletia aboensis has long-standing ethnopharmacological indications for the treatment of symptoms and diseases related to menopause. This investigation examined effects its phytoestrogen-rich fraction on some predictors cardiovascular risk in an ovariectomized rat model In vitro, antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Ovariectomy used trigger menopause by surgically removing ovaries under anesthesia. After recovery, animals were treated orally with (PERF) M. daily 30 days. Blood samples obtained estimation serum lipid profile, enzyme activities, peroxidation, platelet count. The PERF showed strongest inhibition DPPH radical IC50 value 19.65 µg/ml. At 200 400 mg/kg, dose-dependently significant increase high-density lipoprotein (HDL) (42.86 43.05 mg/dl) a (p < 0.05) decrease low-density (LDL) levels (3.78 1.24 compared (OVX) control (31.82 20.32 mg/dl, respectively). Being dose-dependently, increased catalase (CAT) superoxide dismutase (SOD) activities as well significantly reduced malondialdehyde (MDA) OVX control. restored count values (316.54 343.3 103/IU, respectively) close that sham-operated (365.17 103/IU). ability reverse abnormalities associated ovariectomy coupled count, make it potential therapeutic phytoestrogen remedy management complications estrogen deficiency.